Využití paramagnetických částic pro izolaci oligonukleotidů s různou sekvencí a délkou řetězce

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Title in English	Use of paramagnetic particles for the isolation of oligonucleotides with different sequence and chain length
Authors	FOJTÍKOVÁ Martina BARTOŠKOVÁ Jana HOLUBOVÁ Sylvie KALETOVÁ Eva TRNKOVÁ Libuše KIZEK René
Year of publication	2010
Type	Article in Proceedings
Conference	XXX. moderní elektrochemické metody - sborník přednášek
MU Faculty or unit	Faculty of Science
Citation
Field	Biochemistry
Keywords	Paramagnetic particles (MPs); Oligonucleotide; Adenine; Cytosine;Thymine; Adsorptive transfer stripping; Linear sweep voltammetry
Description	Studies of biomolecules strongly depend on efficiency of their isolation from biological materials. Using paramagnetic particles (MPs) represent new method for these purposes. This method is based on hybridization of target molecules on a surface of particles. MPs can be modified with different molecules, for example sequences of thymine or other nucleotides for isolation of nucleic acids. This method is faster and less laborious then commonly used phenol chloroform extraction. Using MPs we studied the isolation of ODN with different sequence and chain length
Related projects:	Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL) Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine