LC-MS/MS Monitoring Human Plasma Proteins Potentionally related to Graft-versus-Host Disease

Warning

This publication doesn't include Faculty of Arts. It includes Faculty of Medicine. Official publication website can be found on muni.cz.

Authors	JEDLIČKOVÁ Lenka BRZOBOHATÝ Jiří KABÁTHOVÁ Jitka VERNER Jan POSPÍŠILOVÁ Šárka ZDRÁHAL Zbyněk
Year of publication	2010
Type	Conference abstract
MU Faculty or unit	Faculty of Medicine
Citation
Description	The aim of this study was to develop a reliable and sensitive method for determination of potential biomarkers in human plasma for early GVHD detection. Blood plasma was immunodepleted by MARS Hu-14 to remove fourteen most abundant proteins. The immunodepleted plasma samples were fractionated by LC capillary system on a reverse phase (mRP C18, Agilent) in water/acetonitrile gradient and subsequently digested with trypsin. The resulting peptides were analyzed using LC-MS/MS in autoMS/MS mode.
Related projects:	Molecular basis of cell and tissue regulations Molecular mechanisms of the cell proliferation and differentiation Změny genomu a proteomu u leukemických pacientů s reakcí štěpu proti hostiteli (GvHD)