LC-MS/MS Monitoring Human Plasma Proteins Potentionally related to Graft-versus-Host Disease

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Authors

JEDLIČKOVÁ Lenka BRZOBOHATÝ Jiří KABÁTHOVÁ Jitka VERNER Jan POSPÍŠILOVÁ Šárka ZDRÁHAL Zbyněk

Year of publication 2010
Type Conference abstract
MU Faculty or unit

Faculty of Medicine

Citation
Description The aim of this study was to develop a reliable and sensitive method for determination of potential biomarkers in human plasma for early GVHD detection. Blood plasma was immunodepleted by MARS Hu-14 to remove fourteen most abundant proteins. The immunodepleted plasma samples were fractionated by LC capillary system on a reverse phase (mRP C18, Agilent) in water/acetonitrile gradient and subsequently digested with trypsin. The resulting peptides were analyzed using LC-MS/MS in autoMS/MS mode.
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