Spektroskopická a elektroforetická studie reaktivity oxidu osmičelého s thymidinmonofosfátem v přítomnosti dusíkatých ligandů

• Title in English: Spectroscopic and electrophoretic study of OsO4 reactivity with TMP in the presence of nitrogen ligands
• Authors: BITTOVÁ Miroslava; POLÁŠEK Miroslav; KOSTEČKA Pavel; MATĚJKA Pavel; TRNKOVÁ Libuše
• Year of publication: 2010
• Type: Article in Proceedings
• Conference: Sborník 14. česko – slovenské spektroskopické konference
• MU Faculty or unit: Faculty of Science
• Field: Biochemistry
• Keywords: osmium tetroxide; thymidine monophosphate; electrophoresis; spectrophotometry; Raman spectroscopy
• Description: Study of hybridization and properties of DNA often uses labeling of bases or short nucleotide sequences with fluorescent, radioactive and electrochemical labels. OsO4 complexes with nitrogen containing ligands belong to the last group. They possess selective affinity to pyrimidine bases, especially thymine. Experimental and theoretical results suggest that bidentate N ligands do not react with OsO4 in water solution. The coordination occurs after OsO4 reduction by addition to thymine double bond but the rate of complexation with TMP differs in the dependence of N ligand. During electrophoretical separation we moreover observed 2 products which possibly correspond to two different diastereoisomers

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