Signalling mechanisms involved in the inhibition of gap-junctional intercellular communication by cyanobacterial extracts
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| Year of publication | 2010 |
| Type | Conference abstract |
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| Description | We investigated effects of various cyanobacterial extracts and pure cyanotoxin microcystin-LR on the inhibition of gap-junctional intercellular communication (GJIC) along with activations of mitogen-activated protein kinases (MAPKs) in normal rat liver stem-like WB-F344 cell line. Modulation of both parameters is an important in vitro biomarker of tumor promotional potencies. Pure microcystin did not inhibit GJIC or activate MAPKs, but complex extracts of water blooms significantly inhibited GJIC and activated MAPK, independent from the content of microcystin. The most pronounced effects were systematically observed in extracts of the cultures of Aphanizomenon flos-aquae as well as in complex water bloom extract dominated by the same species, which does not produce microcystin. Microcystis sp. culture or water bloom had significant but less pronounced effects on both GJIC inhibition and MAPK activation. We have also investigated possible mechanisms controlling the inhibition of GJIC by cyanobacterial extract by using inhibitors of different signaling pathways such as PC-PLC, ERK, PKA and others. |
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