HPLC determination of caffeine and paraxanthine in saliva as a method for CYP1A2 metabolic activity assessment in humans

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Authors	JUŘICA Jan ZENDULKA Ondřej TOMANDL Josef
Year of publication	2010
Type	Conference abstract
MU Faculty or unit	Faculty of Medicine
Citation
Description	The mehod for simple and reliable determination of caffein and paraxanthine in human saliva was developed. After simple, liquid-liquid extraction into organic solvents, sample were separated on Kinetex PFP column and detected at 268 nm. Mobile phase (0.05% acetic acid: methanol, 75:25) was eluted isocratically at flow rate of 0.55 ml/min. All analytes wre eluted within 13 min.
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