Optimalizace separačních podmínek a vývoj metody EMMA pro "in-capillary" studium enzymové aktivity CYP2C9 s diklofenakem

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Title in English	Optimalization of separation conditions and development of EMMA method for in-capillary study of CYP2C9 enzyme activity with diclofenac
Authors	MÁDR Aleš ZEISBERGEROVÁ Marta GLATZ Zdeněk
Year of publication	2010
Type	Article in Proceedings
Conference	Chiranal 2010
MU Faculty or unit	Faculty of Science
Citation
Field	Biochemistry
Keywords	EMMA - cytochrome P450 2C9 - diclofenac - CE - capillary electrophoresis
Description	Capillary electrophoresis offers option to perform either enzymatic or non-enzymatic reactions directly inside capillary. This methodology is termed Electrophoretically Mediated Microanalysis (EMMA) which is respected thanks to indisputable benefits like negligible depletion of valuable reactant or quite simple realization. Cytochromes P450 (CYP) are from pharmacokinetic point of view one of the most studied enzymes, however classic approach for study of enzymatic activity requires relative large amount of enzymes and are not easily automated. For these reasons there was developed EMMA method for study of significant CYP isoform, CYP 2C9. As model substrate was chosen diclofenac.
Related projects:	Proteins in metabolism and interaction of organisms with the environment Integrované bioanalytické technologie pro mikroanalýzy a diagnostiku s využitím LIF a hmotnostní spektrometrie Miniaturized on-line drug metabolism system based on capillary electrophoresis