Recombinant protein production in the methylotrophic yeast Pichia pastoris

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Authors

DADÁKOVÁ Kateřina OBOŘIL Michal KAŠPAROVSKÝ Tomáš LOCHMAN Jan

Year of publication 2011
Type Conference abstract
MU Faculty or unit

Faculty of Science

Citation
Description The expression system of the methylotrophic yeast Pichia pastoris is widely used to produce diverse heterologous proteins for research and industry. P. pastoris can express both intracellular and extracellular proteins at high levels. It can be easily genetically manipulated. Unlike bacteria, yeast can perform many posttranslational protein modifications. These reasons make P. pastoris a suitable system for protein expression. We have optimized the production of recombinant cryptogein in fermenter cultures of P. pastoris. Cryptogein is a small basic protein secreted by pathogenic oomycete Phytophtora cryptogea. It is used to study plant defense reaction. We are able to produce 50-70 mg of this protein during one week fermentation. This work was supported by Grant agency of the Czech Republic (P501/11/1003).
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