Structural analysis of delta-subnit of RNA-polymerase by X-ray

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Authors

DEMO Gabriel PAPOUŠKOVÁ Veronika SANDEROVA Hana WIMMEROVÁ Michaela

Year of publication 2011
Type Conference abstract
MU Faculty or unit

Central European Institute of Technology

Citation
Description RNA polymerase (RNAP) is an essential enzyme that is responsible for transcription of DNA into RNA. The recombinant form of delta-subunit from Bacillus subtilis is 173 aa long protein. The structure of N-terminal domain was determined by NMR. Crystallization and structure determination by X-ray analysis were also chosen. Crystals were obtained after 7 to 9 days with an average size 0.5 mm x 0.4 mm. Diffraction data were collected at ESRF Grenoble, ID 23-1.
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