Biotinylation of quantum dots for application in fluoroimmunoassays with biotin-avidin amplification

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Authors	HLAVÁČEK Antonín BOUCHAL Pavel SKLÁDAL Petr
Year of publication	2012
Type	Article in Periodical
Magazine / Source	Microchimica Acta
MU Faculty or unit	Faculty of Science
Citation
Doi	http://dx.doi.org/10.1007/s00604-011-0729-6
Field	Biochemistry
Keywords	biotin; avidin; albumin; bioconjugation
Description	A competitive microplate fluoroimmunoassay was developed for the determination of human serum albumin in urine. It is based on the use of biotinylated CdTe quantum dots (QDs) whose synthesis is optimised in terms of storage stability, purification, and signal-to-noise ratio. The bioconjugated QDs were characterised by gel chromatography and gel electrophoresis. Storage stability and quantum yield were investigated. The excitation/emission wavelengths are 360/620 nm. The immunoassay of human serum albumin in urine has a working range from 1.7 to 10 ug/mL, and the limit of detection is 1.0 ug/mL.
Related projects:	Proteins in metabolism and interaction of organisms with the environment Biomolecular centre