Bacteriocin genes among E. coli strains: the incidence depends on genotype and phenotype of E. coli strains
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| Year of publication | 2011 |
| Type | Conference abstract |
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| Description | E. coli strains (n = 1670) isolated during years 2007-2010 in the Czech Republic were collected from patients attending regional hospitals in Brno. All strains were tested using commercially available biochemical tests (ENTEROtest 16; Erba Lachema, Ltd., Brno, Czech Republic) for their biochemical activities, and with PCR for the presence to one out of four E. coli phylogroups including group A, B1, B2, and D. Moreover, the incidence of 29 bacteriocin-encoding determinants (i.e. colicin and microcin encoding genes) was tested in each individual E. coli strain. According to their biochemical activity, 1670 E. coli strains clustered into 6 groups containing 709, 50, 47, 744, 103, and 17 strains, respectively. The two largest clusters differed in the incidence of both phylogroups B2 and D and also in the incidence of bacteriocin determinants of mH47, mM, E1 and K. In addition, the incidence of colicinogenic strains was higher in the phylogroup A and B1 (p < 0.001) compared to combined B2 and D phylogroup strains. Strains of phylogroup B2 most frequently encoded microcin H47 and strains of phylogroup D microcin B17 and colicin Js. The incidence of individual bacteriocin encoding determinants is therefore predominantly associated with particular E. coli phylogroups and also with E. coli strains of specific biochemical activity. |
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