RNA

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Authors	ŠTEFL Richard SKLENÁŘ Vladimír
Year of publication	2012
Type	Chapter of a book
MU Faculty or unit	Central European Institute of Technology
Citation
Description	The chapter addresses the current methodologies for RNA investigation by liquid-state NMR spectroscopy. The current technology for the preparation and purification of uniformly and segmentally isotopically labeled RNA is reviewed. An overview of essential spectroscopic strategies for NMR studies of RNA is presented. Critical steps in RNA investigations by NMR are demonstrated by protocols, examples, along with practical advice for troubleshooting.
Related projects:	Biophysics and bioinformatics of genomic DNA fragments very rich in guanine and adenine Strukturní podstata ukončení transkripce nezávislé na poly(A) signálu CEITEC - Central European Institute of Technology Centrum biologie RNA