Project information
New approaches in electrochemical analysis of nucleic acids and oligonucleotides aimed at a ultrasensitive microdetection of DNA and DNA hybridization
- Project Identification
- IAA100040602
- Project Period
- 1/2006 - 12/2008
- Investor / Pogramme / Project type
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Academy of Sciences of the Czech Republic
- Grants of distinctly investigative character focused on the sphere of research pursued at present particularly in the ASCR
- MU Faculty or unit
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Faculty of Science
- prof. RNDr. Libuše Trnková, CSc.
- doc. RNDr. Pavel Janderka, CSc.
- Ing. Radka Mikelová, Ph.D.
- Irena Postbieglová
- Mgr. Roland Rozik, Ph.D.
- Mgr. Jana Topinková
- Mgr. Jaromír Toušek, Dr.
- Keywords
- DNA determination, DNA hybridization, purine derivatives, voltammetry, chronopotentiometry
- Cooperating Organization
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Institute of Biophysics
- Responsible person RNDr. František Jelen, CSc.
The proposed research is dealing with structure, determination and interactions of oligodeoxynucleotides in solution and at surfaces. New important information should be obtained about oligonucleotides and nucleic acids at electrically charged surfaces realized by solid and modifies electrodes. Proposal goals will be achieved using voltammetric and chronopotentiometric methods and their combination with new techniques such as curent elimination and adsorptive transfer stripping. In addition, it is expected that analytical procedures obtained in this project will be utilized in development of simple, sensitive and selective biosensor fo r the electrochemical detection of DNA hybridisation. Highly sensitive methods based on the detection of nucleic acid bases will be developed to find application in various fields of biochemistry and molecular biology.
Publications
Total number of publications: 46
2006
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Resolution of overlapped reduction signals in short hetero-oligonucleotides by elimination voltammetry
11th International conference on electroanalysis, year: 2006
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Současnost a budoucnost eliminačních metod
Eliminační metody I, year: 2006
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Spectrophotometric analysis of nucleic acid bases in binary and ternary mixtures by partial least squares and artificial neural networks
3rd International Symposium on Computer Applications and Chemometrics in Analytical Chemistry SCAC 2006, year: 2006
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Toxicological aspects of flavonoid interaction with biomacromolecules
Neuroendocrinol. Lett., year: 2006, volume: 27, edition: suppl2
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Vliv pH a doby akumulace na elektrochemické chování krátkých hetero-oligonukleotidů
XXVI. Moderní elektrochemické metody, year: 2006
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Využití eliminační voltametrie v proteomice
Eliminační metody I, year: 2006