Dynamic Structures of DNA Heptamers with Different Central Trinucleotide Sequences Studied by Electrochemical and Spectral Methods

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Authors

PILAŘOVÁ Iveta KEJNOVSKÁ Iva VORLÍČKOVÁ Michaela TRNKOVÁ Libuše

Year of publication 2014
Type Article in Periodical
Magazine / Source Electroanalysis
MU Faculty or unit

Faculty of Science

Citation
Web http://onlinelibrary.wiley.com/doi/10.1002/elan.201400288
Doi http://dx.doi.org/10.1002/elan.201400288
Field Physical chemistry and theoretical chemistry
Keywords DNA heptamers; Voltammetry; Elimination voltammetric procedure; Circular dichroism; Polyacrylamide gel electrophoresis
Description A short, single-stranded DNA fragment d(GCGAAGC) and its analogs d(GCNNNGC) carrying different trinucleotide sequences in the center of the molecule (AAA,CCC, GGG or TTT) were investigated by spectral (CD and UV spectra) and electrochemical methods (voltammetry, polyacrylamide gel electrophoresis). Our results show that (i) the central triplet GAA or AAA dramatically stabilizes DNA hairpins, (ii) a stem-loop configuration is not supported by CCC or TTT sequences, instead, these heptamers adopt bimolecular duplex forms. Finally (iii), in the case of GGG sequence a very stable supramolecular G-quadruplex is formed.
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