Eudiplozoon nipponicum (Monogenea): Challenge for next-generation sequencing

• Authors: DVOŘÁKOVÁ Hana; JEDLIČKOVÁ Lucie; KAŠNÝ Martin; ILGOVÁ Jana; BROŽ Pavel; STRNAD Hynek; LEONTOVIČ Roman; ROUDNICKÝ Pavel; VOREL Jiří; DZIKA Ewa; MIKEŠ Libor; GELNAR Milan
• Year of publication: 2015
• Type: Conference abstract
• MU Faculty or unit: Faculty of Science
• Description: Eudiplozoon nipponicum (family Diplozoidae, Polyopisthocotylea) is the hematophagous ectoparasite from the gills of cyprinid fish (Cyprinus carpio). The properties of genes (e.g. identity, structure, evolution) and proteins (e.g. function) of the members from the family Monogenea are among the less investigated in whole phylum Platyhelminthes. Up to now only two genomes and one transcriptome of monogenean species have been sequenced – genome of Protopolystoma xenopodis (Polyopisthocotylea) and Gyrodactylus salaris (Monopisthocotylea) and transcriptome of Neobenedenia melleni. Therefore we adopted the Illumina MiSeq/HiSeq techniques for sequencing the genomic DNA and RNA isolated from adult worms of E. nipponicum. This was followed by comprehensive bioinformatic analysis of sequential data.

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