IMMUNOHISTOCHEMICAL AND IMMUNOGOLD ANALYSIS OF LAMININS IN THE PERIPHERAL NERVE STRUCTURES

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Authors	DUBOVÝ Petr SVÍŽENSKÁ Ivana KLUSÁKOVÁ Ilona GHALIB Nabil Ahmed
Year of publication	2000
Type	Article in Proceedings
Conference	37th Proceeding of Czech Society of Histochemistry and Cytochemistry
MU Faculty or unit	Faculty of Medicine
Citation
Field	Neurology, neurosurgery, neurosciences
Description	The endoneurium and perineurium of femoral nerve displayed immunofluorescence for laminin-1, -2, and -3 isoforms. A granular immunostaining was observed in the endoneurium and perineurium when antibodies against a1 and b2 laminin chains were used. Immunostaining for b3 and g2 laminin chains revealed distinct fluorescence in the Schwann cells, while the perineurium expressed predominantly punctate immunofluorescence. The inner core of Pacinian corpuscles derived from specialized Schwann cells displayed immunofluorescence staining for laminin-1, -2, and -3 as well as for individual laminin chains (a1, b2, b3, g2). In addition, antibodies against a1 and b2 laminin chains provided a weak fluorescence in the axon terminals and superficial layers of the capsule. The results indicate that Schwann cells and their derived structures exhibit not only laminin-1, -2, -3 isoforms but also laminin-5 suggested from immunohistochemical identification of g2 chain.
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