Mapování sekvenčních rozdílů u rodu Treponema.
| Title in English | Mapping of sequencing differences in Treponema genus. |
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| Authors | |
| Year of publication | 2009 |
| Type | Article in Proceedings |
| Conference | XIII. Setkání biochemiků a molekulárních biologů. |
| MU Faculty or unit | |
| Citation | |
| Field | Genetics and molecular biology |
| Keywords | Treponema pallidum; whole genome fingerprinting |
| Description | Genome comparison of 6 treponemal strains including Mexico A, DAL-1 (T. pallidum subsp. pallidum), Gauthier, CDC-2 (T. pallidum subsp. pertenue), Bosnia A (T. pallidum subsp. endemicum) and Fribourg-Blanc (unclassified simian isolate) with the sequenced genome of the syphilis spirochete TPA strain Nichols. The whole genome fingerprinting technique was used for identification of heterologous regions in the chromosomal DNA of analyzed strains. All analyzed strains contained two regions of repetitive sequences (TP0433-434, TP0470) with strain specific repeat numbers. In the yaws and simian strain genomes, 4 specific indels were observed. Strain specific changes comprised e.g. insertion of 58 bp (TP0136) in DAL-1, deletion of 48 bp (TP0548) and insertion of 0.5 kb (TP0695-697) in Fribourg-Blanc. Genomes of T. p. pallidum, T. p. pertenue, T. p. endemicum and unclassified simian isolate are closely related and most of the observed sequence differences are localized in tpr genes and in their vinicity. |
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