Selectivity among Two Lectins: Probing the Effect of Topology, Multivalency and Flexibility of “Clicked” Multivalent Glycoclusters

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Authors

CECIONI Samy FAURE Sophie DARBOST Ulrich BONNAMOUR Isabelle PARROT-LOPEZ Helene ROY Olivier TAILLEFUMIER Claude WIMMEROVÁ Michaela PRALY Jean-Pierre IMBERTY Anne VIDAL Sebastien

Year of publication 2011
Type Article in Periodical
Magazine / Source Chemistry - A European Journal
MU Faculty or unit

Central European Institute of Technology

Citation
Doi http://dx.doi.org/10.1002/chem.201002635
Field Biophysics
Keywords lectin; glycocluster;SPR;ITC
Description We have designed a series of multivalent galactosylated glycoconjugates and studied their binding properties towards two lectins, from plant and bacterial origins, to determine their potential selectivity. The synthesis was achieved through copper(I)-catalysed azide-alkyne cycloaddition (CuAAC) under microwave activation between propargylated multivalent scaffolds and an azido-functionalised carbohydrate derivative. The interactions of two galactose-binding lectins from Pseudomonas aeruginosa (PA-IL) and Erythrina cristagalli (ECA) with the synthesized glycoclusters were studied by hemagglutination inhibition assays (HIA), surface plasmon resonance (SPR) and isothermal titration microcalorimetry (ITC). The results obtained illustrate the influence of the scaffold's geometry on the affinity towards the lectin and also on the relative potency in comparison with a monovalent galactoside reference probe.
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