Selectivity among Two Lectins: Probing the Effect of Topology, Multivalency and Flexibility of “Clicked” Multivalent Glycoclusters

Varování

Publikace nespadá pod Filozofickou fakultu, ale pod Středoevropský technologický institut. Oficiální stránka publikace je na webu muni.cz.

Autoři	CECIONI Samy FAURE Sophie DARBOST Ulrich BONNAMOUR Isabelle PARROT-LOPEZ Helene ROY Olivier TAILLEFUMIER Claude WIMMEROVÁ Michaela PRALY Jean-Pierre IMBERTY Anne VIDAL Sebastien
Rok publikování	2011
Druh	Článek v odborném periodiku
Časopis / Zdroj	Chemistry - A European Journal
Fakulta / Pracoviště MU	Středoevropský technologický institut
Citace
Doi	http://dx.doi.org/10.1002/chem.201002635
Obor	Biofyzika
Klíčová slova	lectin; glycocluster;SPR;ITC
Popis	We have designed a series of multivalent galactosylated glycoconjugates and studied their binding properties towards two lectins, from plant and bacterial origins, to determine their potential selectivity. The synthesis was achieved through copper(I)-catalysed azide-alkyne cycloaddition (CuAAC) under microwave activation between propargylated multivalent scaffolds and an azido-functionalised carbohydrate derivative. The interactions of two galactose-binding lectins from Pseudomonas aeruginosa (PA-IL) and Erythrina cristagalli (ECA) with the synthesized glycoclusters were studied by hemagglutination inhibition assays (HIA), surface plasmon resonance (SPR) and isothermal titration microcalorimetry (ITC). The results obtained illustrate the influence of the scaffold's geometry on the affinity towards the lectin and also on the relative potency in comparison with a monovalent galactoside reference probe.
Související projekty:	Proteiny v metabolismu a při interakci organismů s prostředím Program developing interdisciplinary research POtential for the STudies of BIOMolecular INteractions Návrh antibakteriálních a antivirových léků na bázi cukrů a glykomimetik