A solution study of chemical equilibria of physiologically active nucleobases
| Autoři | |
|---|---|
| Rok publikování | 2013 |
| Druh | Článek ve sborníku |
| Konference | Structure and dynamics of biological macromolecules A biophysical workshop |
| Fakulta / Pracoviště MU | |
| Citace | |
| Obor | Analytická chemie, separace |
| Klíčová slova | DNA;RNA;nucleobase;molecular absorption spectroscopy;UV-VIS;OPIUM; |
| Popis | Nucleobases are building blocks of nucleic acids (DNA, RNA) where they are responsible for the transfer of genetic information. The structure and properties of nucleic acids can be influenced by their protonation and/or their complexation with metal ions [1]. Some structurally modified nucleobases and nucleosides exhibit physiological activity and therefore they are used as drugs [2]. This contribution is focused on the study of protonation constants of adenine-like compounds. They were determined by means of molecular absorption spectroscopy in UV region (I = 0.15 mol.l-1 M NaCl, t = 25 degrees C) and the experimental data were evaluated by OPIUM program. In addition, the temperature and ionic-strength dependence of protonation constants of studied ligands was used to estimate thermodynamic parameters (deltaH0, deltaS0, protonation constant(s) for infinite dilution). In some cases, the luminescence emission spectroscopy was also employed due to significantly high quantum yield of some compounds. Some examples of ligand complexation by zinc metal ion will be presented and their importance for bioligand speciation in vivo will be discussed. |
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