Project information
Proteome dynamics in response to increased cytokinin levels in Arabidopsis
- Project Identification
- IAA600040701
- Project Period
- 1/2007 - 12/2010
- Investor / Pogramme / Project type
-
Academy of Sciences of the Czech Republic
- Grants of distinctly investigative character focused on the sphere of research pursued at present particularly in the ASCR
- MU Faculty or unit
- Faculty of Science
- Keywords
- cytokinins, proteome, protein phosphorylation, Arabidopsis, dexamethasone-inducible gene expression system, 2-D protein electrophoresis, MALDI-TOF/TOF MS
- Cooperating Organization
-
Institute of Biophysics
- Responsible person prof. RNDr. Břetislav Brzobohatý, CSc.
- Responsible person Ing. Janette Bobálová, CSc.
Cytokinins (CKs) are known to act in combination with other plant hormones to regulate diverse developmental processes in plants. The exact molecular mechanisms of CK action in regulation of plant development are the subject of intense current research. While genome expression profiling of CK action has already been initiated by other groups elsewhere, here we propose to analyze proteome dynamics in response to increased CK levels in Arabidopsis under conditions similar to those used in monitoring genome-wide changes in gene expression. This will allow, for the first time, a comparison of the transcriptome and proteome data on highly comparable samples. Increase in CK levels will be achieved by treatment with exogenous CKs and by employing a novel dexamethasone-inducible gene expression system pOp/LhGR. A direct comparison of effects of three distinct CK classes is expected to contribute to elucidation of their individual function(s) in regulation of plant development.
Publications
Total number of publications: 18
2009
-
Early cytokinin response proteins and phosphoproteins of Arabidopsis thaliana identified by proteome and phosphoproteome profiling
Year: 2009, type: Conference abstract
-
Modulation of cytokinin action by decreased intensity of white light in Arabidopsis - a proteomic analysis
XXXIX Annual Meeting of the European Society for New Methods in Agricultural Research: Book of Abstracts, year: 2009
-
Phosphopeptides analysis using enrichment techniques and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
Year: 2009, type: Conference abstract
-
Phosphopeptides analysis using enrichment techniques and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
3rd Central and Eastern European Proteomics Conference. Book of Abstracts, year: 2009
-
Proteomic analysis of A. thaliana influenced by light and cytokinins
Year: 2009, type: Conference abstract
-
Purification and characterization of proteins from plant material using divergent flow isoelectric focusing followed by MALDI MS
Year: 2009, type: Conference abstract
2008
-
A New Approach to Protein Enzymatic Digestion for Fast Protein Identification by Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry
Year: 2008
-
Investigation of the matrix influence on the quality of MALDI-TOF mass spectra of intact proteins and glycoproteins
Year: 2008, type: Conference abstract